Thousands of struggling bank customers will be connected to Indian call centres while more affluent account holders will get one in Britain.
A new banking phone system will identify those with low credit approvals and put them through to India.
Those who have considerable savings or a credit limit that allows them to borrow around £500 from the bank or buy its products will be connected to a British operator.
Bosses hope the new line will filter out their customers who do not have the money to buy the bank's products so their British staff will potentially be able to sell to every caller.
Barclays is testing the system and call centre staff have been briefed that it will start on April 1.
One Barclays sales executive said: 'Before we've had people ringing up who have been overdrawn, so we haven't been able to sell them anything.'
Critics say the bank is only interested in people with cash and not the genuine concerns of millions who have queries about the state of their finances.
Eddy Weatherill of the Independent Banking Advisory Service said: 'This is an apartheid system and certainly preferential to their wealthier customers...Barclays Apatheid Week, anyone?